外泌體萃取分析

Exosome extraction and analysis

E1-外泌體螢光分析儀

ZetaView - Nanoparticle Tracking Analyzer

Zetaview x30

服務項目

• 提供客製化外泌體純化 

• 測量外泌體粒徑大小、分布、濃度及 Zeta 電位

• 提供外泌體特定蛋白檢測

• 檢測外泌體mRNA/miRNA種類及含量

• 外泌體CD marker定量與活性測定

 

管理人員

專案經理：鄭亦棻博士

電子信箱：ifencheng1122@gate.sinica.edu.tw

電話：02-7750-5596

 

專人/專案諮詢 網路預約
外泌體螢光分析儀

機器教育訓練 (自行上機需求) 或專案服務需求，請洽鄭亦棻博士。

 

ZetaView 是一種應用於奈米粒子追踪分析（NTA）的儀器。
這項技術利用雷射光在樣品中反射，透過高解析度的CMOS鏡頭捕捉奈米粒子的布朗運動軌跡，
(1) 並使用Stokes-Einstein 方程式分析粒子的大小和顆粒濃度 (Size & Concentration)。
(2) 在液體靜止形式的樣本中，透過載台兩端的電極以微電泳實驗 (Micro-electrophoresis)，可以間接探究奈米粒子在溶液中的表面電荷的狀態 (Zeta Potential)。
(3) 儀器內置長通濾波片，能夠觀察標有特殊螢光抗體 (Fluorescence antibody) 的奈米粒子，提供更多生物訊息或是更準確的純度數據 。
(4) ZetaView以液體靜止狀態下去偵測奈米粒子的另一優勢是，這樣的模式可以實現螢光共定位分析，用以判斷同一粒子是否成功標定上兩種螢光抗體 (Colocalization)-目前核心共儀提供488nm與647nm螢光共定位分析

Bio-nanoparticles

 - Extracellular Vesicles (EVs)

 - Exosomes

 - Liposomes & Micelles

 - Protein Agglomerates

 - Viruses & Virus-Like-Particles (VLPs)

 - Drug Delivery

 - Fluorescent-labelled NPs

Low concentration samples

 - Nanobubbles

 - Trace Particles

 

放置地點

國家生技研究園區A棟5樓A512室

管理人員

專案經理：鄭亦棻博士

電子信箱：ifencheng1122@gate.sinica.edu.tw

電話：02-7750-5596

 

ZETAVIEW® | NANOPARTICLE TRACKING ANALYSIS INSTRUMENT

The ZetaView® is a unique Nanoparticle Tracking Analysis (NTA) instrument for measuring hydrodynamic particle size, zeta potential, concentration, fluorescence and colocalization

Key Features:

  • Scanning NTA – automated measurements are taken at 11 positions through the sample cell, providing a robust interrogation of samples with powerful statistics, without need for additional accessories.
  • Fixed cell assembly design – allowing easy cleaning with no disassembly required.
  • Increased fluorescence capabilities – a 12- position filter wheel increases the extent to which fluorescently labelled biomarkers can be analysed in one sample.
  • Colocalization –visualisation of colocalized nanoparticles offers the unique ability for NTA to produce data on the percentage of colocalization within a single sample measurement.
  • Auto-alignment & auto-focus – the optical setup is automatically optimized by the software, saving the user time preparing the instrument for use and removing subjective user input bias.
  • Sample recovery – The sample recovery port allows full recovery analysed samples without cross contamination with cleaning buffer.
  • Fast measurements –  The latest software allows for even faster analysis. Over 2,000 particles can be analysed in as little as 60 seconds.
  • Quick and simple cleaning – cleaning between samples requires only a quick flush. The new fixed cell design allows very quick access to the measuring cell with a bayonet lock

  •  

  • APPLICATIONS

  • Extracellular Vesicles (EV) and Exosomes
  • Bio-nanoparticles
  • Liposomes and Micelles
  • Drug Delivery
  • Protein Agglomeration
  • Viruses
  • Virus Like Particles (VLP)
  • Emulsions
  • Polymers
  • Nanometals
  • Nanobubbles
  • Humic Acids
  • Quantum Dots
  • F-lablled Nanoparticles

 

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