外泌體萃取分析

Exosome extraction and analysis

E3-ÄKTA flux 6 半自動 切向流過濾濃縮系統

ÄKTA™ flux 6 tangential flow filtration system

Capacity: 1 L to 20 L

服務項目

• 提供客製化外泌體純化 

• 測量外泌體粒徑大小、分布、濃度及 Zeta 電位

• 提供外泌體特定蛋白檢測

• 檢測外泌體mRNA/miRNA種類及含量

• 外泌體CD marker定量與活性測定

 

管理人員

專案經理：鄭亦棻博士

電子信箱：ifencheng1122@gate.sinica.edu.tw

電話：02-7750-5596

 

ÄKTA flux 6 半自動 切向流過濾濃縮系統

 

目前ÄKTA flux 6 僅提供專案使用‚ 有需求請洽詢鄭亦棻博士

 

儀器名稱

ÄKTA flux 6 切向流過濾濃縮系統

 

 

儀器簡介

ÄKTA flux 6 是一款專為生物製程開發與中至大規模生產應用而設計的切向流過濾（Tangential Flow Filtration, TFF）系統。本系統適用於中高工作體積範圍（1公升至20公升）的樣品處理，能有效執行濃縮、緩衝液置換、細胞收穫與澄清等操作，並可依需求達成不同濃縮倍率，廣泛應用於生物製藥與蛋白質純化流程中。

 

系統採用切向流過濾技術，使樣品液體以平行於濾膜表面的方向流動，進而減少濾膜堵塞現象，並於膜表面產生掃流效應，有效去除累積顆粒物，提升過濾效率與膜材使用壽命。此技術亦可實現濃縮與滲濾（diafiltration）同步進行，簡化操作流程並提升處理效率。

 

 

主要應用領域

  1. 生物樣品之濃縮、過濾、液體置換與去鹽處理
  2. 外泌體、單株抗體與重組蛋白質之回收與純化
  3. 大分子與小分子生物物質之分離
  4. 管柱層析前的樣品預處理步驟
  5. 支援微濾(Microfiltration)與超濾（Ultrafiltration）操作

透過上述功能，可實現連續性過濾、濃縮、分離、去鹽及透析等多步驟純化流程。

 

 

系統規格

  • 操作介面：配備觸控式螢幕，可直覺式操作流速、溫度、壓力等各項參數
  • 系統監控：具即時監控功能，操作中可檢視系統狀態並提供異常警報通知
  • 運作模式：支援恆定體積洗濾與恆定流速操作
  • 輸送幫浦流速：最高可達1000 mL/min
  • 儲液槽容量：最大可達7000 mL，並具備秤重模組與磁力攪拌裝置
  • 溫度操作範圍：介於3°C至39°C之間

 

 

 

放置地點

國家生技研究園區A棟6樓A610室

 

管理人員

專案經理：鄭亦棻博士

電子信箱：ifencheng1122@gate.sinica.edu.tw

電話：02-7750-5596

 

ÄKTA flux 6 Tangential Flow Filtration (TFF) System

 

Instrument Description

 

The ÄKTA flux 6 is a versatile tangential flow filtration system designed for concentration, buffer exchange, cell harvesting, and clarification processes. It is suitable for both process development and large-scale production, capable of handling medium to high working volumes ranging from 1 L to 20 L. The system delivers efficient and scalable filtration, supporting a wide range of concentration factors.

 

Employing Tangential Flow Filtration (TFF), the system allows the feed stream to flow parallel to the membrane surface. This configuration significantly reduces membrane fouling by continuously sweeping away retained particles, thus maintaining high filtration efficiency and extending membrane lifespan. During operation, the system enables concurrent concentration and diafiltration, streamlining workflows and improving process outcomes.

 

 

Key Applications

 

  1. Concentration, filtration, buffer exchange and desalting of biological samples
  2. Recovery and purification of exosomes, antibodies and recombinant proteins
  3. Separation of macromolecules and small biomolecules
  4. Sample preparation prior to column chromatography
  5. Compatible with microfiltration and ultrafiltration techniques

 

This system supports integrated workflows for continuous filtration, concentration, separation, desalting, and dialysis.

 

 

Technical Specifications

 

  • Touchscreen interface for precise control of operational parameters (flow rate, temperature and pressure)
  • Real-time system monitoring with integrated alarm and status display functions
  • Supports both constant volume diafiltration and constant flow rate filtration modes
  • Maximum pump flow rate: 1000 mL/min
  • Reservoir capacity: up to 7000 mL, equipped with weighing function and magnetic stirring
  • Operating temperature: 3°C to 39°C

 

 

Usage Guidelines

Users are required to contact the system administrator prior to use.

 

 

System Location

Room A610, 6th Floor, Building A

National Biotechnology Research Park

 

 

Contact Information

Technical Specialist: Dr. I-Fen Cheng

Email: ifencheng1122@gate.sinica.edu.tw

Phone: +886-2-7750-5596

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