外泌體萃取分析
Exosome extraction and analysis
E2-ÄKTA flux S 手動 切向流過濾濃縮系統
Minimate™ tangential flow filtration system
Capacity: 50 mL -1 L
服務項目
• 提供客製化外泌體純化
• 測量外泌體粒徑大小、分布、濃度及 Zeta 電位
• 提供外泌體特定蛋白檢測
• 檢測外泌體mRNA/miRNA種類及含量
• 外泌體CD marker定量與活性測定
管理人員
專案經理：鄭亦棻博士
電子信箱：ifencheng1122@gate.sinica.edu.tw
電話：02-7750-5596
ÄKTA flux S 提供自行操作使用‚ 請洽詢鄭亦棻博士預約教育訓練及後續認證時段
儀器簡介
ÄKTA flux S system 是一款專為製程開發及小規模生產而設計的切向流過濾（Tangential Flow Filtration, TFF）系統。本系統適用的體積範圍介於（50毫升至1公升）的樣品處理，能有效執行濃縮、緩衝液置換、細胞收穫與澄清等操作，並可依需求達成不同濃縮倍率，廣泛應用於生物製藥與蛋白質純化流程中。
系統採用切向流過濾技術，使樣品液體以平行於濾膜表面的方向流動，進而減少濾膜堵塞現象，並於膜表面產生掃流效應，有效去除累積顆粒物，提升過濾效率與膜材使用壽命。此技術亦可實現濃縮與滲濾（Diafiltration）同步進行，簡化多步驟操作流程，提升效能與重現性。
主要應用領域
- 生物樣品之濃縮、過濾、液體置換與去鹽處理
- 外泌體、單株抗體與重組蛋白質之回收與純化
- 大分子與小分子生物物質之分離
- 管柱層析前的樣品預處理步驟
- 支援微濾（Microfiltration）與超濾（Ultrafiltration）操作
透過上述功能，可實現連續性過濾、濃縮、分離、去鹽及透析等多步驟純化流程。
系統規格
- 操作模式：手動調整操作參數，包括過濾壓力與流速設定
- 進樣幫浦（蠕動式）流速範圍：20~350 mL/min
- 輸送幫浦（蠕動式）流速範圍：1~50 mL/min
- 儲液槽容量：最大容積 500 mL，配備高秤重模組與磁力攪拌功能，以維持樣品均質性與操作穩定性
使用須知
本系統採使用者自行操作制。首次使用者須事先聯繫管理人員，完成必要的教育訓練與使用認證後方可進行操作。
放置地點
國家生技研究園區A棟6樓A610室
管理人員
專案經理：鄭亦棻博士
電子信箱：ifencheng1122@gate.sinica.edu.tw
電話：02-7750-5596
ÄKTA flux S System – Tangential Flow Filtration (TFF) Concentration System
Instrument Description
The ÄKTA flux S system is a tangential flow filtration (TFF) system specifically designed for process development and small-scale production. It is optimized for sample volumes ranging from 50 mL to 1 L, and is well-suited for applications such as concentration, buffer exchange, cell harvesting, and clarification. This system enables efficient processing across various concentration factors.
Utilizing tangential flow filtration technology, the system allows liquids to flow parallel to the membrane surface. This configuration significantly reduces membrane fouling and creates a sweeping flow across the membrane, effectively removing accumulated particulates. The result is enhanced filtration efficiency and extended membrane lifespan. The system also supports simultaneous concentration and diafiltration, simplifying multi-step workflows and improving overall process efficiency and reproducibility.
Key Applications
- Concentration, filtration, buffer exchange and desalting of biological samples
- Recovery and purification of exosomes, monoclonal antibodies and recombinant proteins
- Separation of macromolecules and small biomolecules
- Pre-treatment of samples prior to column chromatography
- Compatible with both microfiltration and ultrafiltration processes
These capabilities enable integrated and continuous workflows for filtration, concentration, separation, desalting, and dialysis in downstream bioprocessing.
Technical Specifications
- Operation Mode: Manual adjustment of filtration parameters, including pressure and flow rate
- Feed Pump (peristaltic): Flow rate range of 20–350 mL/min
- Recirculation Pump (peristaltic): Flow rate range of 1–50 mL/min
- Reservoir Capacity: Up to 500 mL, equipped with a weighing module and magnetic stirring to ensure sample homogeneity and operational stability
User Guidelines
This system is designated for self-operation. First-time users are required to contact the system administrator in advance to complete the mandatory training and certification before operating the instrument.
System Location
Room A610, 6th Floor, Building A
National Biotechnology Research Park
Contact Information
System Administrator: Dr. I-Fen Cheng
Email: ifencheng1122@gate.sinica.edu.tw
Phone: +886-2-7750-5596